Investigating the Antibacterial and Antitumoral Activities of New Copper(II) Complexes with Trifluoromethyluracil Isomers
- Author(s)
- Gabriele de Menezes Pereira, Julia Helena Bormio Nunes, Silmara Cristina Lazarini Frajácomo, Wilton Rogério Lustri, Fernando Rodrigues Goulart Bergamini, Josélia Cristina de Oliveira Moreira, Robson Pontes, Ana Lucia T.G. Ruiz, João Ernesto de Carvalho, Wdeson Pereira Barros, Pedro Paulo Corbi
- Abstract
New copper(II) complexes, derived from the isomers 5-(trifluoromethyl)uracil (L1) and 6-(trifluoromethyl)uracil (L2) with 2,2′-bipyridine (Bpy), [Cu(H2O)(L1)2Bpy] and [Cu(H2O)(L2)2Bpy], were synthesized, characterized, and evaluated for their antibacterial and antitumoral properties. Chemical analyses suggest the molecular formula CuC20H12F6N6O4·H2O for both complexes. Infrared spectroscopic analyses indicate that trifluoromethyluracil isomers coordinate to copper(II) by the oxygen atoms. Structural confirmation of the [Cu(H2O)(L2)2Bpy] complex was obtained by single crystal X-ray diffraction, where two L2 molecules coordinate to the copper(II) ion by both oxygen atoms from carbonyl groups. The complexes demonstrated antibacterial activity against Gram-positive Staphylococcus aureus and Bacillus cereus, and Gram-negative Pseudomonas aeruginosa and Escherichia coli strains. The [Cu(H2O)(L1)2Bpy] complex exhibited the most promising results against P. aeruginosa, with a minimum inhibitory concentration (MIC) value of 0.478 mmol L−1. The [Cu(H2O)(L1)2Bpy] complex demonstrated antiproliferative effects on U251 and MCF-7 cells, with GI50 values (concentration that inhibits 50% of cell growth) of 2.18 ± 1.17 and 16.00 ± 10.07 µmol·L−1, whereas the [Cu(H2O)(L2)2Bpy] complex inhibited the growth of FaDu and U251 cells (GI50 values 4.70 ± 2.69 µmol·L−1 and 9.06 ± 3.86 µmol·L−1, respectively). Neither the precursors nor the copper(II) complexes affected the structure of CT-DNA at the evaluated concentrations, suggesting that DNA is not the main cellular target of the complexes.
- Organisation(s)
- Department of Inorganic Chemistry
- External organisation(s)
- Universidade Estadual de Campinas, University of Araraquara, Universidade Federal de Uberlândia
- Journal
- ChemistrySelect
- Volume
- 10
- ISSN
- 2365-6549
- DOI
- https://doi.org/10.1002/slct.202405096
- Publication date
- 02-2025
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 104003 Inorganic chemistry, 301904 Cancer research
- Keywords
- ASJC Scopus subject areas
- General Chemistry
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/0349a735-7cd0-49e2-9df4-3cdbfe9e7ca2