Effects of Salinomycin and Deferiprone on Lead-Induced Changes in the Mouse Brain

Author(s)
Emilia Petrova, Yordanka Gluhcheva, Ekaterina Pavlova, Ivelin Vladov, Peter Dorkov, Martin Schaier, Irena Pashkunova-Martic, Thomas H. Helbich, Bernhard Keppler, Juliana Ivanova
Abstract

Lead (Pb) is a highly toxic heavy metal that has deleterious effects on the central nervous system. This study aimed to investigate the effects of salinomycin (Sal) and deferiprone (DFP) on brain morphology and on the content of some essential elements in Pb-exposed mice. Adult male Institute of Cancer Research (ICR) mice were exposed to a daily dose of 80 mg/kg body weight (b.w.) Pb(II) nitrate for 14 days and subsequently treated with Sal (16 mg/kg b.w.) or DFP (19 mg/kg b.w.) for another 14 days. At the end of the experimental protocol, the brains were processed for histological and inductively coupled plasma mass spectrometry (ICP-MS) analyses. Pb exposure resulted in a 50-fold increase in Pb concentration, compared with controls. Magnesium (Mg) and phosphorus (P) were also significantly increased by 22.22% and 17.92%, respectively. The histological analysis of Pb-exposed mice revealed brain pathological changes with features of neuronal necrosis. Brain Pb level remained significantly elevated in Sal- and DFP-administered groups (37-fold and 50-fold, respectively), compared with untreated controls. Treatment with Sal significantly reduced Mg and P concentrations by 22.56% and 18.38%, respectively, compared with the Pb-exposed group. Administration of Sal and DFP ameliorated brain injury in Pb-exposed mice and improved histological features. The results suggest the potential application of Sal and DFP for treatment of Pb-induced neurotoxicity.

Organisation(s)
Department of Analytical Chemistry, Department of Inorganic Chemistry
External organisation(s)
BIOVET JSC, Medizinische Universität Wien, Sofia University "St. Kliment Ohridski", Bulgarian Academy of Sciences (BAS)
Journal
International Journal of Molecular Sciences
Volume
24
ISSN
1661-6596
DOI
https://doi.org/10.3390/ijms24032871
Publication date
02-2023
Peer reviewed
Yes
Austrian Fields of Science 2012
106023 Molecular biology, 301407 Neurophysiology, 104003 Inorganic chemistry
Keywords
ASJC Scopus subject areas
Catalysis, Molecular Biology, Spectroscopy, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry, Inorganic Chemistry
Sustainable Development Goals
SDG 3 - Good Health and Well-being
Portal url
https://ucrisportal.univie.ac.at/en/publications/07100cba-7ebf-4e55-82e2-768ec81b5baf