Rapid, high-yield enzymatic synthesis of n.c.a. 6-[<sup>18</sup>F]fluorodopamine (6-[<sup>18</sup>F]FDA) for in vivo application

Author(s)
Karsten Bamminger, Julia Raitanen, Georgios Karanikas, Sazan Rasul, Lukas Nics, Markus Mitterhauser, Wolfgang Wadsak, Marcus Hacker, Verena Pichler, Chrysoula Vraka
Abstract

6-[18F]Fluorodopamine (6-[18F]FDA) proved valuable as a diagnostic tool for neuroendocrine and rare tumors, such as ganglioneuromas and pheochromocytomas, however, clinical application is still limited. So far, its radiosyntheses were impeded by the formation of side-products, low molar activity, multi-step reactions and the use of challenging precursors. Here, we describe a detailed enzymatic procedure for fast, simple and high yield synthesis of no carrier added 6-[18F]FDA from 6-[18F]FDOPA with high radiochemical yield of ~69 % and >95 % purity as determined by radio-HPLC and -TLC. The product formulation was stable and suitable for in vivo application.

Organisation(s)
Department of Pharmaceutical Sciences
External organisation(s)
Medizinische Universität Wien, Centre for Biomarker Research in Medicine – CBmed GmbH, Ludwig Boltzmann Intitute Applied Diagnostics
Journal
Nuclear Medicine and Biology
Volume
114-115
Pages
189-197
No. of pages
9
ISSN
0969-8051
DOI
https://doi.org/10.1016/j.nucmedbio.2022.07.001
Publication date
2022
Peer reviewed
Yes
Austrian Fields of Science 2012
104020 Radiochemistry
Keywords
ASJC Scopus subject areas
Molecular Medicine, Radiology Nuclear Medicine and imaging, Cancer Research
Sustainable Development Goals
SDG 3 - Good Health and Well-being
Portal url
https://ucrisportal.univie.ac.at/en/publications/27c21448-cf30-477e-90af-09715dd5903d