The labelling of Nanocoll(R) with [111In] for dual-isotope scanning
- Author(s)
- Markus Mitterhauser, Wolfgang Wadsak, Leonhard-Key Mien, Harald Eidherr, Sebastian Roka, Georg Zettinig, Peter Angelberger, Helmut Viernstein, Kurt Kletter, Robert Dudczak
- Abstract
Visualization and biopsy of sentinel lymph nodes play an important role in planning and controlling the therapy of breast cancer. Hitherto two methods - scintigraphy or gamma probe detection after injection of [99mTc]- nanocolloids and visual detection after injection of patent blue dye - are used routinely. There are no conclusive publications elucidating such important parameters as injection site, injection method and colloidal parameters. The present work aims to label Nanocoll� with [111In] to provide an alternative method, a simultanous one-compound dual-isotope application. Methods: [111In]-Indiumchloride was buffered with acetate and transferred to the nanocolloid. The colloid labelling reaction was complete after 30min and filtrated through 100nm Nuclepore� filters. Results: Incorporation yield of [111In]-Indium into the nanocolloid was nearly quantitative, the step associated with the major loss of activity was the particle sizing with a mean yield of 55%. Conclusion: The presented method allows for the routine supply of [111In]-nanocolloids. Size-filtered [111In]-Nanocoll� shows the same particle size range as [ 99mTc]-Nanocoll�. Œ 2003 Elsevier Ltd. All rights reserved.
- Organisation(s)
- Department of Inorganic Chemistry, Department of Nutritional Sciences, Department of Sport and Human Movement Science
- External organisation(s)
- Allgemeines Krankenhaus der Stadt Wien (AKH), Austrian Research Centers GmbH (ARC Seibersdorf)
- Journal
- Applied Radiation and Isotopes
- Volume
- 59
- Pages
- 337-342
- No. of pages
- 6
- ISSN
- 0969-8043
- Publication date
- 2007
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 301208 Pharmaceutical technology
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/2a3f871a-0476-4451-b97e-247b26feb54e