G-quadruplex vs. duplex-DNA binding of nickel(II) and zinc(II) Schiff base complexes
- Author(s)
- Riccardo Bonsignore, Alessio Terenzi, Angelo Spinello, Annamaria Martorana, Antonino Lauria, Anna Maria Almerico, Bernhard K. Keppler, Giampaolo Barone
- Abstract
Novel nickel(II) (1) and zinc(II) (2) complexes of a Salen-like ligand, carrying a pyrimidine ring on the N,N′ bridge, were synthesized and characterized. Their interaction with duplex and G-quadruplex DNA was investigated in aqueous solution through UV-visible absorption, circular dichroism and viscometry measurements. The results obtained point out that, while the zinc(II) complex does not interact with both duplex and G-quadruplex DNA, the nickel(II) complex 1 binds preferentially to G-quadruplex respect to duplex-DNA, with values of the DNA-binding constants, K
b, 2.6 × 10
5 M
- 1 and 3.5 × 10
4 M
- 1, respectively. Molecular dynamics simulations provided an atomic level model of the top-stacking binding occurring between 1 and hTelo (a 22-mer sequence oligonucleotide) in G-quadruplex conformation.
- Organisation(s)
- Department of Inorganic Chemistry
- External organisation(s)
- Università degli Studi di Palermo, Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Medizinische Universität Wien
- Journal
- Journal of Inorganic Biochemistry
- Volume
- 161
- Pages
- 115-121
- No. of pages
- 7
- ISSN
- 0162-0134
- DOI
- https://doi.org/10.1016/j.jinorgbio.2016.05.010
- Publication date
- 08-2016
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 104003 Inorganic chemistry, 106002 Biochemistry
- Keywords
- ASJC Scopus subject areas
- Biochemistry, Inorganic Chemistry
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/375cafcf-600b-49d2-88ee-00f19eea33d8