Quantitative bioimaging by LA-ICP-MS

Author(s)

Alexander E. Egger, Sarah Theiner, Christoph Kornauth, Petra Heffeter, Walter Berger, Bernhard K. Keppler, Christian Hartinger

Abstract

Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was used to study the spatially-resolved distribution of ruthenium and platinum in viscera (liver, kidney, spleen, and muscle) originating from mice treated with the investigational ruthenium-based antitumor compound KP1339 or cisplatin, a potent, but nephrotoxic clinically-approved platinum-based anticancer drug. Method development was based on homogenized Ru- and Pt-containing samples (22.0 and 0.257 μg g^-1, respectively). Averaging yielded satisfactory precision and accuracy for both concentrations (3-15% and 93-120%, respectively), however when considering only single data points, the highly concentrated Ru sample maintained satisfactory precision and accuracy, while the low concentrated Pt sample yielded low recoveries and precision, which could not be improved by use of internal standards (¹¹⁵In, ¹⁸⁵Re or ¹³C). Matrix-matched standards were used for quantification in LA-ICP-MS which yielded comparable metal distributions, i.e., enrichment in the cortex of the kidney in comparison with the medulla, a homogenous distribution in the liver and the muscle and areas of enrichment in the spleen. Elemental distributions were assigned to histological structures exceeding 100 μm in size. The accuracy of a quantitative LA-ICP-MS imaging experiment was validated by an independent method using microwave-assisted digestion (MW) followed by direct infusion ICP-MS analysis.

Organisation(s)

Department of Inorganic Chemistry, Department of Functional Materials and Catalysis

External organisation(s)

Medizinische Universität Wien, University of Auckland

Journal

Metallomics: integrated biometal science

Volume

6

Pages

1616-1625

No. of pages

10

ISSN

1756-5901

DOI

https://doi.org/10.1039/c4mt00072b

Publication date

09-2014

Peer reviewed

Yes

Austrian Fields of Science 2012

104003 Inorganic chemistry, 301904 Cancer research, 106037 Proteomics

Keywords

ASJC Scopus subject areas

Metals and Alloys, Chemistry (miscellaneous), Biophysics, Biochemistry, Biomaterials

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Portal url

https://ucrisportal.univie.ac.at/en/publications/2ea730d8-07c3-4f3c-9faf-d6359a95ea4f