The heterocyclic ruthenium(III) complex KP1019 (FFC14A) causes DNA damage and oxidative stress in colorectal tumor cells
- Author(s)
- S Kapitza, Michael Jakupec, Maria Uhl, Bernhard Keppler, Brigitte Marian
- Abstract
The Ru(III) complex salt KP1019 induced formation of H2O2 in colorectal tumor cells in a dose-dependent way. It also caused DNA-strand breaks if only weakly doubling tail length to 55.87?3.97 mikroM. Both effects were prevented by 5 mM N-acetylcysteine (NAC) which also reduced cytotoxicity (IC50 55 vs 30 mikroM without NAC). Induction of apoptosis was shown by loss of mitochondrial membrane potential in 63.4?2.1% of the population and by caspase-dependent cleavage of poly-(ADP-ribose)-polymerase (PARP). Both effects were inhibited by NAC which reduced the population with depolarized mitochondrial membranes to 24.1?1.2% and prevented PARP-cleavage indicating a central role oxidative stress in KP1019-induced apoptosis. ? 2005 Elsevier Ireland Ltd. All rights reserved.
- Organisation(s)
- Department of Inorganic Chemistry
- External organisation(s)
- Medizinische Universität Wien
- Journal
- Cancer Letters
- Volume
- 226
- Pages
- 115-121
- No. of pages
- 7
- ISSN
- 0304-3835
- Publication date
- 2005
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 301904 Cancer research
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/d73db8a5-9fdd-40ca-857d-ab6a7f179e6c