Optical tweezers directed one-bead one-sequence synthesis of oligonucleotides
- Author(s)
- Tao Wang, Stefan Oehrlein, Mark Manuel Somoza, Jose R. Sanchez Perez, Ryan Kershner, Franco Cerrina
- Abstract
An optical tweezers directed parallel DNA oligonucleotide synthesis methodology is described in which controlled pore glass (CPG) beads act as solid substrates in a two-stream microfluidic reactor. The reactor contains two parallel sets of physical confinement features that retain beads in the reagent stream for synthetic reaction but allow the beads to be optically trapped and transferred between the reagent and the inert streams for sequence programming. As a demonstration, we synthesized oligonucleotides of target sequence 25-nt, one deletion and one substitution using dimethoxytrityl (DMT) nucleoside phosphoramidite chemistry. In detecting single-nucleotide mismatches, fluorescence in situ hybridization of the bead-conjugated probes showed high specificity and signal-to-noise ratios. These preliminary results suggest further possibilities of creating a novel type of versatile, sensitive and multifunctional reconfigurable one-bead one-compound (OBOC) bead array.
- Organisation(s)
- Department of Inorganic Chemistry
- External organisation(s)
- University of Notre Dame, University of Wisconsin, Madison
- Journal
- Lab on a Chip - Miniaturisation for Chemistry, Biology and Bioengineering
- Volume
- 11
- Pages
- 1629-1637
- No. of pages
- 9
- ISSN
- 1473-0197
- DOI
- https://doi.org/10.1039/C0LC00577K
- Publication date
- 2011
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 104003 Inorganic chemistry
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/6748e55b-b709-40d4-9cb4-6ed6339ba185