Capillary electrophoretic study of carboplatin and analogues with nucleoside monophosphates, di- and trinucleotides

Author(s): Angelika Kueng, Andrea Zenker, Mathea Sophia Galanski, Bernhard Keppler
Abstract: Carboplatin (cis-diammine-1,1-cyclobutane-dicarboxylatoplatinum(II)) is the only cisplatin (cis-diammine-dichloroplatinum(II)) derivative currently available for the treatment of cancer worldwide. The higher stability of the carboxylate ligand compared to the coordinated chloride in cisplatin results in a reduced reactivity of the molecule. Capillary electrophoresis has been applied for investigating the adduct formation of carboplatin and analogues with nucleoside monophosphates, di- and trinucleotides. Adduct formation results in a significant shift of the absorption maximum to lower energy compared to free nucleotides. Therefore, characterization of the analytes was performed by UV additionally to NMR spectroscopy. A preference for GMP- and AMP-coordination was found. The ability of separating all four common nucleotides and their major platinum adducts in a single run demonstrates the suitability of CE for this kind of investigations. Copyright Œ 2001 Elsevier Science B.V.
Organisation(s): Department of Inorganic Chemistry
External organisation(s): University of Vienna
Journal: Journal of Inorganic Biochemistry
Volume: 83
Pages: 181-186
No. of pages: 6
ISSN: 0162-0134
DOI: https://doi.org/10.1016/S0162-0134(00)00182-3
Publication date: 2001
Peer reviewed: Yes
Austrian Fields of Science 2012: 1040 Chemistry
Sustainable Development Goals: SDG 3 - Good Health and Well-being
Portal url: https://ucrisportal.univie.ac.at/en/publications/78382983-5523-4ff2-b0e0-452cbd3593de