Ruthenium versus platinum: Interactions of anticancer metallodrugs with duplex oligonucleotides characterised by electrospray ionisation mass spectrometry
- Author(s)
- Michael Groessl, Yury O. Tsybin, Christian Hartinger, Bernhard Keppler, Paul J. Dyson
- Abstract
The binding of the ruthenium-based anticancer drug candidates KP1019, NAMI-A and RAPTA-T towards different double-stranded oligonucleotides was probed by electrospray ionisation mass spectrometry and compared with that of the widely used platinum-based chemotherapeutics cisplatin, carboplatin and oxaliplatin. It was found that the extent of adduct formation decreased in the following order: cisplatin > oxaliplatin > NAMI-A > RAPTA-T > carboplatin > KP1019. In addition to the characterisation of the adducts formed with the DNA models, the binding sites of the metallodrugs on the oligonucleotides were elucidated employing top-down tandem mass spectrometry and were found to be similar for all the metallodrugs studied, irrespective of the sequence of the oligonucleotide. A strong preference for guanine residues was established.
- Organisation(s)
- Department of Inorganic Chemistry
- External organisation(s)
- École polytechnique fédérale de Lausanne
- Journal
- Journal of Biological Inorganic Chemistry
- Volume
- 15
- Pages
- 677-688
- No. of pages
- 12
- ISSN
- 0949-8257
- DOI
- https://doi.org/10.1007/s00775-010-0635-0
- Publication date
- 2010
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 104003 Inorganic chemistry, 1030 Physics, Astronomy, 301303 Medical biochemistry
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/7ff77084-87c4-4a8b-b5d7-486eccc18c5c