Fragmentation methods on the balance: unambiguous top-down mass spectrometric characterization of oxaliplatin-ubiquitin binding sites
- Author(s)
- Samuel Matthias Meier, Yury O. Tsybin, Paul J. Dyson, Bernhard Keppler, Christian Hartinger
- Abstract
The interaction between oxaliplatin and the model protein ubiquitin (Ub) was investigated in a top-down approach by means of high-resolution electrospray ionization mass spectrometry (ESI-MS) using diverse tandem mass spectrometric (MS/MS) techniques, including collision-induced dissociation (CID), higher-energy C-trap dissociation (HCD), and electron transfer dissociation (ETD). To the best of our knowledge, this is the first time that metallodrug-protein adducts were analyzed for the metal-binding site by ETD-MS/MS, which outperformed both CID and HCD in terms of number of identified metallated peptide fragments in the mass spectra and the localization of the binding sites. Only ETD allowed the simultaneous and exact determination of Met1 and His68 residues as binding partners for oxaliplatin. CID-MS/MS experiments were carried out on orbitrap and ion cyclotron resonance (ICR)-FT mass spectrometers and both instruments yielded similar results with respect to number of metallated fragments and the localization of the binding sites. A comparison of the protein secondary structure with the intensities of peptide fragments generated by collisional activation of the [Ub + Pt-(chxn)] adduct [chxn = (1R,2R)-cyclohexanediamine] revealed a correlation with cleavages in solution phase random coil areas, indicating that the N-terminal beta-hairpin and alpha-helix structures are retained in the gas phase.
- Organisation(s)
- Department of Inorganic Chemistry
- External organisation(s)
- École polytechnique fédérale de Lausanne
- Journal
- Analytical and Bioanalytical Chemistry
- Volume
- 402
- Pages
- 2655-2662
- No. of pages
- 8
- ISSN
- 1618-2642
- Publication date
- 2012
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106002 Biochemistry, 1030 Physics, Astronomy
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/863d62c7-1f2b-4892-9bd5-bdb661fc0155