Application of capillary electrophoresis-mass spectrometry for the investigation of the binding behavior of oxaliplatin to 5'-GMP in the presence of the sulfur-containing amino acid-L-methionine
- Author(s)
- Dirk B. Strickmann, Angelika Kueng, Bernhard Keppler
- Abstract
Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) has been used for investigating the influence of the sulfur containing amino acid L-methionine (L-Met) on the binding behavior of oxaliplatin (trans-R,R-diaminocyclohexane-(oxalato) platinum(II)) to 5'-GMP. L-Methionine competes with 5'-GMP for the platinum binding site and forms as well as 5'-GMP adducts with oxaliplatin. The formation of the prognosed complexes [Pt(DACH)(L-Met-S,N)]+ and [Pt(DACH)(5'-GMP)2]2- (DACH = 1,2-diaminocyclohexane) could be proved directly by using CE-ESI-MS. Furthermore, we could now bring forward proofs, that the coordination of 5'-GMP with oxaliplatin is inhibited by L-methionine and could show, that the 5'-GMP ligands of the [Pt(DACH) (5'-GMP)2]2- complex can be replaced slowly by L-methionine whereas methionine can not be replaced by GMP.
- Organisation(s)
- Department of Inorganic Chemistry
- External organisation(s)
- University of Vienna
- Journal
- Electrophoresis
- Volume
- 23
- Pages
- 74-80
- No. of pages
- 7
- ISSN
- 0173-0835
- DOI
- https://doi.org/10.1002/1522-2683(200201)23:1<74::AID-ELPS74>3.0.CO;2-F
- Publication date
- 2002
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 1040 Chemistry
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/c12a3bde-2d8d-4a2d-b079-a54e1daa7445