Reaction monitoring of platinum(II) complex-5'-guanosine monophosphate adduct formation by ion exchange liquid chromatography/electrospray ionization mass spectrometry
- Author(s)
- Peter Zöllner, Andrea Zenker, Mathea Sophia Galanski, Bernhard Keppler, Wolfgang Lindner
- Abstract
Cisplatin and four structurally related platinum(II) complexes were incubated with guanosine 5'-monophosphate (5'-GMP) in water at 37°C. The adduct formation reactions were monitored with cation-and anion-exchange liquid chromatography/electrospray ionization mass spectrometry. In addition to mono- and bis-adducts of guanosine 5'-monophosphate with the platinum(II) complexes, other molecular species, presumably with a binuclear structure (two platinum(II) centres), were detected in the reaction mixtures, which have not been reported previously, indicating an unexpected complexity of adduct formation. Anion-exchange chromatography revealed the presence of isomers of two complexes which presumably result from the restricted rotation at the platinum-N-7 (5'-GMP) bonds. All reaction products were characterized in both the positive and negative ion modes. Furthermore, preliminary kinetics and half-times of complex formation were investigated for cisplatin and two other platinum(II) complexes, monitoring the relative concentrations of free 5'-GMP and of mono- and bis-GMP adducts as a function of time (250 h) using an internal standard protocol with thymidine 5'-monophosphate. Copyright Œ 2001 John Wiley & Sons, Ltd.
- Organisation(s)
- Department of Analytical Chemistry, NMR Centre, Department of Inorganic Chemistry
- External organisation(s)
- University of Vienna
- Journal
- Journal of Mass Spectrometry
- Volume
- 36
- Pages
- 742-753
- No. of pages
- 12
- ISSN
- 1076-5174
- DOI
- https://doi.org/10.1002/jms.178
- Publication date
- 2001
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 1040 Chemistry
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/db5065ef-cdf8-4229-99ee-e00e628d14af