HPIC-UV-ICP-SFMS study of the interaction of cisplatin with guanosine monophosphate
- Author(s)
- Stephan Hann, Andrea Zenker, Mathea Sophia Galanski, Thomas Bereuter, Gerhard Stingeder, Bernhard Keppler
- Abstract
Interaction of cis-[Pt(NH3)2Cl2] (cisplatin) with 5'-guanosine monophosphate (5'-GMP) has been investigated for the first time by on-line coupling of high performance ion chromatography (HPIC) to inductively coupled plasma sector field mass spectrometry (ICP-SFMS). The time-dependent reaction course of the cisplatin-5'-GMP system was followed after incubation under simulated physiological conditions by monitoring the decrease in the concentration of 5'-GMP and the increase in the concentration of formed adducts, on the basis of speciation analysis. Because of the two-step mechanism an intermediate mono adduct was observed together with the major product, the bis adduct c;s-[Pt(NH3)2(GMP)2]2-. The data obtained correlated well with those from earlier studies employing orthogonal techniques such as capillary electrophoresis (CE). Furthermore, HPIC-ICP-SFMS provided unambiguous stoichiometric information about the major GMP-adduct. For this purpose the platinum-to-phosphorus ratio was determined by simultaneously measuring 31P and 195Pt. To separate significant interferences from 15N16O+, 14N16O1H+, 12C18O1H+, and 13C17O1H+ on 31P, high-mass resolution (m/?m = 4500) proved to be mandatory. The P/Pt signal ratio of 2/1 obtained corresponds to the molar ratio in the bis adduct m-[Pt(NH3)2(GMP)2]2-. ΠSpringer-Verlag 2001.
- Organisation(s)
- Department of Inorganic Chemistry
- External organisation(s)
- University of Natural Resources and Life Sciences, Agilent Technologies Deutschland GmbH
- Journal
- Fresenius' Journal of Analytical Chemistry
- Volume
- 370
- Pages
- 581-586
- No. of pages
- 6
- ISSN
- 0937-0633
- DOI
- https://doi.org/10.1007/s002160100740
- Publication date
- 2001
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 1040 Chemistry
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/dce7661d-5a57-4a94-80cc-28d93a2dade0