Nonaqueous Oxidation in DNA Microarray Synthesis Improves the Oligonucleotide Quality and Preserves Surface Integrity on Gold and Indium Tin Oxide Substrates
- Author(s)
- Erika Schaudy, Glenda Gisela Ibanez Redin, Etkin Parlar, Mark M. Somoza, Jory Lietard
- Abstract
Nucleic acids attached to electrically conductive surfaces are very frequently used platforms for sensing and analyte detection as well as for imaging. Synthesizing DNA on these uncommon substrates and preserving the conductive layer is challenging as this coating tends to be damaged by the repeated use of iodine and water, which is the standard oxidizing medium following phosphoramidite coupling. Here, we thoroughly investigate the use of camphorsulfonyl oxaziridine (CSO), a nonaqueous alternative to I2/H2O, for the synthesis of DNA microarrays in situ. We find that CSO performs equally well in producing high hybridization signals on glass microscope slides, and CSO also protects the conductive layer on gold and indium tin oxide (ITO)-coated slides. DNA synthesis on conductive substrates with CSO oxidation yields microarrays of quality approaching that of conventional glass with intact physicochemical properties.
- Organisation(s)
- Department of Inorganic Chemistry
- External organisation(s)
- Technische Universität München
- Journal
- Analytical Chemistry
- Volume
- 96
- Pages
- 2378-2386
- No. of pages
- 9
- ISSN
- 0003-2700
- DOI
- https://doi.org/10.1021/acs.analchem.3c04166
- Publication date
- 02-2024
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 104002 Analytical chemistry, 204001 Inorganic chemical technology
- ASJC Scopus subject areas
- Analytical Chemistry
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/e3d380be-2972-4488-85e2-284ed3776633